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Quantitative analysis of retinol and retinol palmitate in vitamin tablets using ^1H-nuclear magnetic resonance spectroscopy [An article from: Analytica Chimica Acta]
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Quantitative analysis of retinol and retinol palmitate in vitamin tablets using ^1H-nuclear magnetic resonance spectroscopy [An article from: Analytica Chimica Acta]

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Editorial Review: This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

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^1H-NMR spectrometry was applied to the quantitative analysis of Vitamin A in four different types of vitamin tablets without any chromatographic purification or saponification. The experiment was performed analysing the H-15 resonance, which appears at @d 4.32 for retinol and @d 4.69 for retinol palmitate, well separated from other resonances in the ^1H-NMR spectrum. Compounds were quantified using the relative ratio of the integral of the H-15 signal to that of a known amount of internal standard (200@mg/ml), anthracene. In order to evaluate the feasibility of avoiding the saponification of retinol palmitate in the preparation of samples, several solvents such as dimethylsulfoxide, n-hexan...

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